Conventional PCR systems require users to set a predetermined number of cycles based on assay- and sample-specific factors. When performing single-cell sequencing experiments, this requires knowledge ...
Quantitative polymerase chain reaction (qPCR) is a molecular technique that has reshaped the landscape of biological research and medicine due to its high sensitivity and specificity. qPCR, also known ...
What is real-time quantitative PCR? qPCR uses one of two methods to detect the changes to DNA amplification: use of fluorescent dyes or sequence-specific DNA probes. qPCR is normally performed in a ...
Journal of Plant Pathology, Vol. 97, No. 2 (July 2015), pp. 259-263 (5 pages) Leifsonia (Clavibacter) xyli subsp. xyli (Lxx) is the etiological agent of ratoon stunting disease (RSD), one of the most ...
PCR involves three core components: polymerase, primer, and template. Primers anneal to templates and create initiation sites for the polymerase. The polymerase then recruits free deoxynucleoside ...
Quantitative PCR (qPCR) is a widely utilized technique for the semiquantitative study of gene expression. However, its accuracy and reproducibility depend significantly on the proper use of assay ...
Researchers at Columbia Engineering and Rover Diagnostics, both at Columbia University, New York, NY, have teamed up to develop a low-cost, photonics-based portable platform that gives RT-PCR results ...
A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost with an 'auto-pilot' mindset. However, how many secrets are hidden ...
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